Antibodies can engage specific receptors at the surface of effector cells and mediate several functions beyond viral neutralization. Increasing evidence suggest that Fc-mediated effector functions, such as antibody dependent cellular cytotoxicity (ADCC), have an important role in protection against SARS-CoV-2 infection. We engineered a cell line stably expressing a GFP-tagged SARS-CoV-2 Spike to measure ADCC. This protocol provides an optimized way of measuring ADCC activity mediated by anti-SARS-CoV-2 Spike monoclonal antibodies or plasma from previously infected or vaccinated individuals.

Fuente: STAR Protocols
Available online 13 September 2021, 100851
In Press, Journal Pre-proof