Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a novel highly virulent coronavirus that causes severe illness and mortality in certain patients. SARS-CoV-2 contains a positive-sense single-stranded RNA genome that encodes two essential cysteine proteases, a papain-like (PLpro) and 3C-like main protease (3CLpro). These proteases cleave the nonstructural polyproteins essential for viral maturation and replication.1, 2 Recently, much interest has been focused on identifying novel protease inhibitors against SARS-CoV-2.3-7 The essentiality of SARS-CoV-2 viral cysteine proteases prompted us to test a pharmacological inhibitor of the cysteine protease calpain-1 against recombinant 3CLpro and SARS-CoV-2 infection in mammalian cells…

Fuente: Journal of Cellular and Molecular Medicine

First published: 09 September 2022